Morphology, growth, and physiological traits of greenhouse cucumber seedlings as affected by supplementary white and blue LEDs

Zhengnan Yan, Long Wang, Jie Cheng, Duo Lin, Yanjie Yang

Abstract


Supplemental lighting can be applied in the greenhouse to obtain high-quality seedlings when the solar daily light integral (DLI) is insufficient. However, there is no optimal strategy for the supplementary light provided by white and blue light-emitting diodes (LEDs) with the same DLI in cucumber (Cucumis sativus L.) seedling production grown in the greenhouse in early spring. The objective of the study was to determine changes in morphology, photosynthesis, growth, and physiological characteristics in greenhouse-grown cucumber seedlings (cv. Tianjiao No. 5) depending on different supplementary fractions (28.5%, 33.5%, 38.5%, 43.5%, and 48.5%) of blue light (B) under constant DLI provided by combinations of white (B28.5% included) and blue LEDs, and cucumber seedlings were grown with sunlight only were set as the control. The results documented that supplementary light resulted in compact and robust greenhouse-grown cucumber seedlings with higher chlorophyll content and net photosynthetic rate compared to those grown without supplementary light. The plant height and hypocotyl length of cucumber seedlings decreased quadratically with an increase of blue light fractions provided by combinations of white and blue LEDs. Additionally, the leaf area and stem diameter of cucumber seedlings increased first and a decreased trend was observed subsequently with the increasing fraction of blue light in a quadratic function. Similar trends were found in root architecture (e.g., root length, root surface area, and root volume) and root activity of cucumber seedlings; however, no significant differences were exhibited as blue light fraction increased from 38.5% to 43.5% provided by supplementary light. Stem firmness and cellulose content increased by 26.2% and 23.4%, respectively, as 15% blue light was added to white LEDs. In conclusion, the 43.5% blue light created by supplementary broad-spectrum white and blue LEDs resulted in compact and stoutest cucumber seedlings along with well-developed root system and higher stem firmness, thus improving the mechanical strength of the greenhouse-grown cucumber seedlings for transplanting.
Keywords: blue light, hypocotyl length, photosynthetic capacity, stem firmness, supplementary light
DOI: 10.25165/j.ijabe.20221506.7351

Citation: Yan Z N, Wang L, Cheng J, Lin D, Yang Y J. Morphology, growth, and physiological traits of greenhouse cucumber seedlings as affected by supplementary white and blue LEDs. Int J Agric & Biol Eng, 2022; 15(6): 60–66.

Keywords


blue light, hypocotyl length, photosynthetic capacity, stem firmness, supplementary light

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References


Liu M C, Ji Y H, Wu Z H, He W M. Current situation and development trend of vegetable seedling industry in China. China Veg., 2018; 11: 1–7. (in Chinese)

FAO. Crops and livestock products. 2022. Available: http://www.fao.org/faostat/en/#data/QC. Accessed on [2022-03-19]

Hernández R, Kubota C. Tomato seedling growth and morphological responses to supplemental LED lighting red: Blue ratios under varied daily solar light integrals. Acta. Hort., 2012; 956: 187–194.

Wei H, Hu J, Liu C, Wang M, Zhao J, Kang D, et al. Effect of supplementary light source on quality of grafted tomato seedlings and expression of two photosynthetic genes. Agronomy, 2018; 8(10): 207. doi: 10.3390/agronomy8100207.

Yan Z N, Wang L, Wang Y F, Chu Y Y, Lin D, Yang Y J. Morphological and physiological properties of greenhouse-grown cucumber seedlings as influenced by supplementary light-emitting diodes with same daily light integral. Horticulturae, 2021; 7(10): 361. doi: 10.3390/horticulturae7100361.

Qian M, Rosenqvist E, Flygare A M, Kalbina I, Ke S. UV-A light induces a robust and dwarfed phenotype in cucumber plants (Cucumis sativus L.) without affecting fruit yield. Sci. Hort., 2020; 263: 109110. doi: 10.1016/j.scienta.2019.109110.

Jeong H W, Lee H R, Kim H M, Kim H M, Seung J H. Using light quality for growth control of cucumber seedlings in closed-type plant production system. Plants, 2020; 9(5): 639. doi: 10.3390/plants9050639.

Wei H, Wang M Z, Jeong B R. Effect of supplementary lighting duration on growth and activity of antioxidant enzymes in grafted watermelon seedlings. Agronomy, 2020; 10: 337. doi: 10.3390/agronomy10030337.

Paucek I, Appolloni E, Pennisi G, Quaini S, Gianquinto G, Orsini F. LED Lighting systems for horticulture: business growth and global distribution. Sustainability, 2020; 12(18): 7516. doi: 10.3390/su17187516.

Wei H, Zhao J, Hu J T, Jeong B R. Effect of supplementary light intensity on quality of grafted tomato seedlings and expression of two photosynthetic genes and proteins. Agronomy, 2019; 9(6): 339. doi: 10.3390/agronomy9060339.

He J, Jawahir N, Qin L. Quantity of supplementary LED lightings regulates photosynthetic apparatus, improves photosynthetic capacity and enhances productivity of cos lettuce grown in a tropical greenhouse. Photosynthesis Research, 2021; 149(1): 187–199.

Nozue H, Shirai K, Kajikawa K, Gomi M. White LED light with wide wavelength spectrum promotes high-yielding and energy-saving indoor vegetable production. Acta Hortic, 2018; 1227: 585–592.

Liu N, Ji F, Xu L J, He D X. Effects of led light quality on the growth of pepper seedling in plant factory. Int. J. Agric. & Biol. Eng., 2019; 12(5): 44–50.

Lin K H, Huang M Y, Huang W D, Hsu M H, Yang C M. The effects of red, blue, and white light-emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata). Sci. Hort., 2013, 150: 86–91.

Park Y, Runkle E S. Spectral effects of light-emitting diodes on plant growth, visual color quality, and photosynthetic photon efficacy: White versus blue plus red radiation. PLoS One, 2018; 13(8): 1–14.

Elkins C, Iersel M W V. Longer photoperiods with the same daily light integral improve growth of rudbeckia seedlings in a greenhouse. HortScience, 2020; 55(10): 1676–1682.

Ren X, Liu Y, Jeong H K, Jeong B R. Supplementary light source affects the growth and development of Codonopsis lanceolata seedlings. Int. J. Mol. Sci., 2018; 19(10): 3074. doi: 10.3390/ijms19103074.

Brazaityte A, Miliauskiene J, Vaštakait V, Sutuliene R, Laužike k, Duchovskis P, et al. Effect of different ratios of blue and red led light on brassicaceae microgreens under a controlled environment. Plants, 2021; 10(4): 801. doi: 10.3390/plants10040801.

Paradiso R, Proietti S. Light quality manipulation to control plant growth and photomorphogenesis in greenhouse horticulture: The state of the art and the opportunities of modern LED systems. J. Plant Growth Recul., 2021; 41: 742–780.

Snowden M C, Cope K R, Bugbee B. Sensitivity of seven diverse species to blue and green light: Interactions with photon flux. PLoS One, 2016; 11(10): e0163121. doi: 10.1371/journal.pone.0163121.

Liang Y, Kang C, Kaiser E, Kuang Y, Li T. Red/blue light ratios induce morphology and physiology alterations differently in cucumber and tomato. Sci. Hortic., 2021; 281: 109995. doi: 10.1016/j.scienta.2021.109995.

Hammock H A, Kopsell D A, Sams C E. Supplementary blue and red LED narrowband wavelengths improve biomass yield and nutrient uptake in hydroponically grown basil. HortScience, 2020; 55(12): 1888–1897.

Cope K R, Snowden M C, Bugbee B. Photobiological interactions of blue light and photosynthetic photon flux: Effects of monochromatic and broad-spectrum light sources. photochem Photobiol, 2014; 90(3): 574–584.

Clavijo-Herrera J, Santen E V, Gómez C. Growth, water-use efficiency, stomatal conductance, and nitrogen uptake of two lettuce cultivars grown under different percentages of blue and red light. Horticulturae, 2018; 4(3): 16. doi: 10.3390/horticulturae4030016.

Wang J, Lu W, Tong Y, Yang Q C. Leaf Morphology, photosynthetic performance, chlorophyll fluorescence, stomatal development of lettuce (Lactuca sativa L.) exposed to different ratios of red light to blue light. Front. Plant Sci., 2016; 7: 250. doi: 10.3389/fpls.2016.00250.

Azad M, Kjaer K H, Adnan M, Naznin M T, Lim J D, Sung I J, et al. The Evaluation of growth performance, photosynthetic capacity, and primary and secondary metabolite content of leaf lettuce grown under limited irradiation of blue and red led light in an urban plant factory. Agriculture, 2020; 10(28): e10020028. doi: 10.3390/agriculture10020028.

Ying Q, Jones-Baumgardt C, Zheng Y, Bozzo G. The proportion of blue light from light-emitting diodes alters microgreen phytochemical profiles in a species-specific manner. HortScience, 2020; 56(1): 13–20.

Pennisi G, Blasioli S, Cellini A, Lorenzo M, Andrea C, Ilaria B, et al. Unraveling the role of red: Blue led lights on resource use efficiency and nutritional properties of indoor grown sweet basil. Front. Plant Sci., 2019; 10: 305. doi: 10.3389/fpls.2019.00305.

Chen X L, Xue X Z, Guo W Z, Wang L C, Qiao X J. Growth and nutritional properties of lettuce affected by mixed irradiation of white and supplemental light provided by light-emitting diode. Sci. Hort., 2016; 200: 56(1): 111–118.

Dou H, Niu G, Gu M, Masabni J G. Responses of sweet basil to different daily light integrals in photosynthesis, morphology, yield, and nutritional quality. HortScience, 2018; 53(4): 496–503.

Updegraff D M. Semi-micro determination of cellulose in biological materials. Anal Biochem, 1969; 32(3): 420–424.

Li H S. Principle and technology of plant physiological and biochemical experiments. Higher Education Press, 2003; pp. 119–120.

Hernández R, Kubota C. Growth and morphological response of cucumber seedlings to supplemental red and blue photon flux ratios under varied solar daily light integrals. Sci. Hort., 2014; 173: 92–99.

Palmitessa O D, Paciello P, Santamaria P. Supplemental LED increases tomato yield in mediterranean semi-closed greenhouse. Agronomy, 2020; 10(9): 1353. doi: 10.3390/agronomy10091353.

Larsen D H, Woltering E J, Nicole C C S, Leo F M. Response of basil growth and morphology to light intensity and spectrum in a vertical farm. Front. Plant Sci., 2020; 11: 597906. doi: 10.3389/fpls.2020.597906.

Ji F, Wei S Q, Liu N, Yang P. Growth of cucumber seedlings in different varieties as affected by light environment. Int J Agric & Biol Eng, 2020; 13(5): 73–78.

Yan Z N, He D X, Niu G, Zhou Q, Qu Y. Growth, nutritional quality, and energy use efficiency of hydroponic lettuce as influenced by daily light integrals exposed to white versus white plus red light-emitting diodes. HortScience, 2019; 54(10): 1737–1744.

He D X, Yan Z N, Sun X, Yang P. Leaf development and energy yield of hydroponic sweetpotato seedlings using single-node cutting as influenced by light intensity and LED spectrum. J Plant Physiol, 2020; 254(6):153274. doi: 10.1016/j.jplph.2020.153274.

Hernández R, Eguchi T, Deveci M, Kubota C. Tomato seedling physiological responses under different percentages of blue and red photon flux ratios using LEDs and cool white fluorescent lamps. Sci. Hortic., 2016; 213: 270–280.

Matsuo S, Nanya K, Imanishi S, Honda I, Goto E. Effects of blue and red lights on gibberellin metabolism in tomato seedlings. The Horticulture J., 2019; 88(1): 76–82.

Wang Y F, Chu Y Y, Wan Z, Zhang G, Liu L, Yan Z N. Root architecture, growth and photon yield of cucumber seedlings as influenced by daily light integral at different stages in the closed transplant production system. Horticulturae, 2021; 7: 328. doi: 10.3390/horticulturae7090328.

Lu N, Bernardo E L, Tippayadarapanich C, Michiko T, Natsuko K, Wataru Y. Growth and accumulation of secondary metabolites in perilla as affected by photosynthetic photon flux density and electrical conductivity of the nutrient solution. Front. Plant Sci., 2017; 8: 708. doi: 10.3389/fpls.2017.00708.

Hogewoning S W, Trouwborst G, Maljaars H, Poorter H, Ieperen W, Harbinson J. Blue light dose responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light. J Exp. Bot., 2010; 61: 3107–3117.

Kang W H, Park J S, Park K S, Son J E. Leaf photosynthetic rate, growth, and morphology of lettuce under different fractions of red, blue, and green light from light-emitting diodes (LEDs). Hortic. Environ. Biotechnol., 2016; 57: 573–579.

Kramchote S, Glahan S. Effects of LED supplementary lighting and NPK fertilization on fruit quality of melon (Cucumis Melo L.) grown in plastic house. J. Hortic. Research, 2020; 28(1): 111–122.

Li C, Liu D, Li L, Hu S, Xu Z, Tang C. Effects of light-emitting diodes on the growth of peanut plants. Agron. J., 2018; 110(6): 2369–2377.

Li H, Tang C, Xu Z. The effects of different light qualities on rapeseed (Brassica napus L.) plantlet growth and morphogenesis. Vitro. Sci. Hortic., 2013; 150: 117–124.

Gómez C, Mitchell C. Growth responses of tomato seedlings to different spectra of supplemental lighting. Hortscience, 2015; 50(1): 112–118.

Hitz T, Graeff-Hnninger S, Munz S. Modelling of soybean (Glycine Max (L.) Merr.) response to blue light intensity in controlled environments. Plants, 2020; 9(12): 1757. doi: 10.3390/plants9121757.

Szczesniak A S. Texture is a sensory property. Food Quality & Preference, 2002; 13(4): 215–225.




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