Optimizing a nitrogen-supplemented, condensed corn soluble medium for growth of the Polyhydroxyalkanoate producer Pseudomonas putida KT217

Jeremy Javers, William Gibbons, Chinnadurai Karunanithy

Abstract


Pseudomonas putida KT217 produces medium-chain-length polyhydroxyalkanoate (mcl-PHA) that is of commercial interest as a biodegradable plastic. To reduce PHA production costs, a less expensive medium to grow P. putida KT217 to a high cell dry weight (CDW) was developed. P. putida KT217 was grown in aerated shake flasks on a condensed corn solubles (CCS) based medium that provided several organic acids and carbohydrates that were utilized for growth. The medium was prepared by adding various amounts of fresh CCS (100-600 g/L, or 34.9-209.4 g/L dry basis) to water and then centrifuging and filtering. The CCS permeate media contained dry matter levels of 28.8-164.9 g/L. The CCS permeate medium containing 108.4 g/L solids produced approximately 6 g CDW/L, at a growth rate of 1.03 per hour, and maximum cell population of 4

Keywords


cell density, cell dry weight (CDW), biodegradable plastic, biocreator, pH, nitrogen supplement, growth rate

Full Text:

Provisional PDF PDF

References


Klinke S, Dauner M, Scott G, Kessler B, Witholt B. Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly (3-hydroxyalkanoates) in Pseudomonas puitda. Applied and Environmental Microbiology, 2000; 66(3): 909-913.

Choi J I, Lee S Y. Process analysis and economic evaluation for poly (3-hydroxybutyrate) production by fermentation. Bioprocess Engineering, 1997; 17: 335-342.

Hejazi P, Vasheghani-Farahani E, Yamini Y. Supercritical fluid disruption of Ralstonia eutropha for poly (B-hydroxybutyrate) recovery. Biotechnology Progress, 2003; 19: 1519-1523.

Yu P H F, Chua H, Huang A L, Lo W H, Ho K P. Transformation of industrial food wastes into polyhydroxyalkanoates. Water Science and Technology, 1999; 40(1): 365-370.

Yu J. Production of PHA from starchy wastewater via organic acids. Journal of Biotechnology, 2001; 86: 105- 112.

Koller M, Bona R, Braunegg G, Hermann C, Horvat P, Kroutil M, et al. Production of polyhydroxyalkanoates from agricultural waste and surplus materials. Biomacromolecules, 2005; 6: 561-565.

Ribera R G, Monteoliva-Sanchez M, Ramos-Cormenzana A. Production of polyhidroxyalkanoates by Pseudomonas putida KT2442 harboring pSK2665 in wastewater from olive oil mills (alpechin). Electronic Journal of Biotechnology, 2001; 4(2): 116-119.

Vidal-Mas J, Resina-Pelfort O, Haba E, Comas J, Manresa A, Vives-Rego J. Rapid flow cytometry – Nile red assessment of PHA cellular content and heterogeneity in cultures of Pseudomonas aeruginosa 47T2 (NCIB 40044) grown in waste frying oil. Antoine van Leeuwenhoek, 2001; 80: 57-63.

Du G, Yu J. Green technology for conversion of food scraps to biodegradeable thermoplastic polyhydroxyalkanoates. Environmental Science and Technology, 2002; 36: 5511-5516.

Du G, Chen L X L, Yu J. High-efficiency production of bioplastics from biodegradeable organic solids. Journal of Polymers and the Environment, 2004; 12(2): 89-94.

Keenan T M, Tanenbaum S W, Stipanovic A J, Nakas J P. Production and characterization of poly-B-hydroxyalkanoate copolymers from Burkholderia cepacia utilizing xylose and levulinic acid. Biotechnology Progress, 2004; 20: 1697- 1704.

Gouda M K, Sellam A E, Omar S H. Production of PHB by a Bacillus megaterium strain usin gsugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological Research, 2001; 156: 201-207.

Purushothaman M, Anderson R K I, Narayana S, Jayaraman V K. Industrial byproducts as cheaper medium components influencing the production of polyhydroxyalkanoates (PHA)-biodegradeable plastics. Bioprocess and Biosystem Engineering, 2001; 24: 131-136.

Bock S A, Fox S L, Gibbons W R. Development of a low cost, industrially suitable medium for production of acetic acid from glucose by Clostridium thermoaceticum. Biotechnology and Applied Biochemistry, 1997; 25: 117- 125.

Fosmer A, Gibbons W R, Heisel N. Scleroglucan production from Sclerotium glucanicum on a condensed corn solubles medium. Journal of Biotechnology Research, 2010; 2: 131-143.

Smith R L, West T P, Gibbons W R. Rhodospirillum rubrum: Utilization of condensed corn solubles for poly-(3- hydroxybutyrate-co-3-hydroxyvalerate) production. Journal of Applied Microbiology, 2008; 104: 1488-1494.

Vanderhoff A, Gibbons W R, Bauer N, West T P. Development of a low-cost medium for producing gellan from Sphingomonas paucimobilis. Journal of Biotech Research, 2010; 2: 67-78.

Wolf-Hall C E, Gibbons W R, Bauer N A. Development of a low-cost medium for production of nisin from Lactococcus lactis subsp. lactis. World Journal of Microbiology and Biotechnology, 2009; 25: 2013-2019.

Tan I K P, Kumar K S, Theanmalar M, Gan S N, Gordon III B. Saponified palm kernel oil and its major free fatty acids as carbon substrates for the production of polyhydroxyalkanoates in Pseudomonas putida PGA1. Applied Microbiology Biotechnology, 1997; 47: 207-211.




Copyright (c)



2023-2026 Copyright IJABE Editing and Publishing Office