Progress in microalgae cultivation photobioreactors and applications in wastewater treatment: A review

Han Ting, Lu Haifeng, Ma Shanshan, Yuanhui Zhang, Liu Zhidan, Duan Na


Using microalgae to treat wastewater has received growing attention in the world because it is regarded as a novel means for wastewater treatment. It is commonly recognized that large-scale cultivation and commercial application of microalgae are limited by the development of photobioreactor (PBR). Although there are a lot of PBRs for microalgae pure cultivation which used culture medium, specialized PBRs designed for wastewater treatment are rare. The composition of wastewater is quite complicated; this might cause a very different photosynthetic effect of microalgae compared to those grown in a pure cultivation medium. Therefore, PBRs for wastewater treatment need to be redesigned and improved based on the existing PBRs that are used for microalgae pure cultivation. In this review, different PBRs for microalgae cultivation and wastewater treatment are summarized. PBR configurations, PBR design parameters and types of wastewater are presented. In addition, the wastewater treatment efficiency and biomass productivity were also compared among each type of PBRs. Moreover, some other promising PBRs are introduced in this review, and a two-stage cultivation mode which combines both closed and open system is discussed as well. Ultimately, this article focuses on current problems and gives an outlook for this field, aiming at providing a primary reference for microalgae cultivation by using wastewater.
Keywords: microalgae cultivation, wastewater treatment, photobioreactor (PBR), review
DOI: 10.3965/j.ijabe.20171001.2705

Citation: Han T, Lu H F, Ma S S, Zhang Y H, Liu Z D, Duan N. Progress in microalgae cultivation photobioreactors and applications in wastewater treatment: A review. Int J Agric & Biol Eng, 2017; 10(1): 1–29.


microalgae cultivation, wastewater treatment, photobioreactor (PBR), review


Razzak S A, Hossain M M, Lucky R A, Bassi A S, de lasa H. Integrated CO2 capture, wastewater treatment and biofuel production by microalgae culturing-A review. Renewable & Sustainable Energy Reviews, 2013; 27: 622–653.

Jegathese S J P, Farid M. Microalgae as a renewable source of energy: A niche opportunity. Journal of Renewable Energy, 2014; 2014: 1–10.

Singh N K, Dhar D W. Microalgae as second generation biofuel. A review. Agronomy for Sustainable Development, 2011; 31(4): 605–629.

Bahadar A, Khan M B. Progress in energy from microalgae: A review. Renewable & Sustainable Energy Reviews, 2013; 27: 128–148.

Zeng X, Guo X, Su G, Danquah MK, Zhang S, Lu Y, et al. Bioprocess considerations for microalgal-based wastewater treatment and biomass production. Renewable and Sustainable Energy Reviews, 2015; 42: 1385–1392.

Singh R N, Sharma S. Development of suitable photobioreactor for algae production - A review. Renewable & Sustainable Energy Reviews, 2012; 16(4): 2347–2353.

Gupta P L, Lee S M, Choi H J. A mini review: photobioreactors for large scale algal cultivation. World J Microbiol Biotechnol, 2015; 31(9): 1409–1417.

Fernández F G A, Sevilla J M F, Grima E M. Photobioreactors for the production of microalgae. Reviews in Environmental Science and Bio-Technology, 2013; 12(2): 131–151.

Whitton R, Ometto F, Pidou M, Jarvis P, Villa R, Jefferson B. Microalgae for municipal wastewater nutrient remediation: mechanisms, reactors and outlook for tertiary treatment. Environmental Technology Reviews, 2015; 4(1): 133–148.

Prajapati S K, Kaushik P, Malik A, Vijay V K. Phycoremediation coupled production of algal biomass, harvesting and anaerobic digestion: Possibilities and challenges. Biotechnology Advances, 2013; 31(8): 1408–1425.

Munoz R, Guieysse B. Algal-bacterial processes for the treatment of hazardous contaminants: a review. Water Res, 2006; 40(15): 2799–2815.

Burlew J S. Book reviews: algal culture from laboratory to pilot plant. Algal Culture, 1953; 600(1): 49–50.

ran N, Bartlett J, Kannangara G, Milev A, Volk H, Wilson M. Catalytic upgrading of biorefinery oil from micro-algae. Fuel, 2010; 89(2): 265–274.

Vonshak A, Richmond A. Mass production of the blue-green alga Spirulina: an overview. Biomass, 1988; 15(4): 233–47.

Lee Y K. Microalgal mass culture systems and methods: their limitation and potential. Journal of applied phycology, 2001; 13(4): 307–315.

Benemann J R, Oswald W J. Systems and economic analysis of microalgae ponds for conversion of carbon dioxide to biomass (Final Report: Grant No. DEFG22 93PC93204). Final report, Office of Scientific & Technical Information Technical Reports, 1996.

Oswald W J, Goleuke C. Large-scale production of algae. California Univ., Berkeley (USA), 1967.

Tredici M R. Mass production of microalgae: photobioreactors. Handbook of microalgal culture: Biotechnology and applied phycology, 2004; 1: 178–214.

Vasumathi K K, Premalatha M, Subramanian P. Parameters influencing the design of photobioreactor for the growth of microalgae. Renewable and Sustainable Energy Reviews, 2012; 16(7): 5443–5550.

Wang S K, Stiles A R, Guo C, Liu C Z. Microalgae cultivation in photobioreactors: An overview of light characteristics. Engineering in Life Sciences, 2014; 14(6): 550–559.

Hoffmann J P. Wastewater treatment with suspended and nonsuspended algae. Journal of Phycology, 2002; 34(5): 757–763.

Yadavalli R, Rao C S, Rao R S, Potumarthi R. Dairy effluent treatment and lipids production by Chlorella pyrenoidosa and Euglena gracilis: study on open and closed systems. Asia-Pacific Journal of Chemical Engineering, 2014; 9(3): 368–373.

Sfez S, Van Den Hende S, Taelman S E, De Meester S, Dewulf J. Environmental sustainability assessment of a microalgae raceway pond treating aquaculture wastewater: From up-scaling to system integration. Bioresour Technol, 2015; 190: 321–331.

Posadas E, Morales M D M, Gomez C, Acién F G, Muñoz R. Influence of pH and CO2 source on the performance of microalgae-based secondary domestic wastewater treatment in outdoors pilot raceways. Chemical Engineering Journal, 2015; 265: 239–248.

Hena S, Fatimah S, Tabassum S. Cultivation of algae consortium in a dairy farm wastewater for biodiesel production. Water Resources and Industry, 2015; 10: 1–14.

Tan X, Chu H, Zhang Y, Yang L, Zhao F, Zhou X. Chlorella pyrenoidosa cultivation using anaerobic digested starch processing wastewater in an airlift circulation photobioreactor. Bioresour Technol, 2014; 170: 538–548.

Riano B, Molinuevo B, Garcia-Gonzalez M C. Treatment of fish processing wastewater with microalgae-containing microbiota. Bioresour Technol, 2011; 102(23): 10829–10833.

Tuantet K, Temmink H, Zeeman G, Janssen M, Wijffels R H, Buisman C J N. Nutrient removal and microalgal biomass production on urine in a short light-path photobioreactor. Water Research, 2014; 55: 162–174.

Caporgno M P, Taleb A, Olkiewicz M, Font J, Pruvost J, Legrand J, et al. Microalgae cultivation in urban wastewater: Nutrient removal and biomass production for biodiesel and methane. Algal Research, 2015; 10: 232–239.

Tercero E A R, Sforza E, Morandini M, Bertucco A. cultivation of Chlorella protothecoides with urban wastewater in continuous photobioreactor: biomass productivity and nutrient removal. Applied Biochemistry and Biotechnology, 2014; 172(3): 1470–1485.

Yan C, Zhang Q, Xue S, Sun Z, Wu X, Wang Z, et al. A novel low-cost thin-film flat plate photobioreactor for microalgae cultivation. Biotechnology and Bioprocess Engineering, 2016; 21(1): 103–109.

Choi H J, Lee S M. Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater. Bioprocess Biosyst Eng, 2015; 38(4): 761–766.

Choi H J. Effect of optical panel distance in a photobioreactor for nutrient removal and cultivation of microalgae. World J Microbiol Biotechnol, 2014; 30(7): 2015–2023.

Xue S, Zhang Q, Wu X, Yan C, Cong W. A novel photobioreactor structure using optical fibers as inner light source to fulfill flashing light effects of microalgae. Bioresour Technol, 2013; 138: 141–147.

Ruiz J, Alvarez-Diaz P D, Arbib Z, Garrido-Perez C, Barragan J, Perales J A. Performance of a flat panel reactor in the continuous culture of microalgae in urban wastewater: Prediction from a batch experiment. Bioresour Technol, 2013; 127: 456–463.

Hu B, Zhou W, Min M, Du Z, Chen P, Ma X, et al. Development of an effective acidogenically digested swine manure-based algal system for improved wastewater treatment and biofuel and feed production. Applied Energy, 2013; 107: 255–263.

Min M, Wang L, Li Y, Mohr M J, Hu B, Zhou W, et al. Cultivating Chlorella sp. in a pilot-scale photobioreactor using centrate wastewater for microalgae biomass production and wastewater nutrient removal. Applied Biochemistry and Biotechnology, 2011; 165(1): 123–137.

Solovchenko A, Pogosyan S, Chivkunova O, Selyakh I, Semenova L, Voronova E, et al. Phycoremediation of alcohol distillery wastewater with a novel Chlorella sorokiniana strain cultivated in a photobioreactor monitored on-line via chlorophyll fluorescence. Algal Research, 2014; 6: 234–241.

Zhu L, Wang Z, Shu Q, Takala J, Hiltunen E, Feng P, et al. Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. Water Research, 2013; 47(13): 4294–4302.

Yang C, Ding Z, Zhang K. Growth of Chlorella pyrenoidosa in wastewater from cassava ethanol fermentation. World Journal of Microbiology & Biotechnology, 2008; 24(12): 2919–2925.

Michels M H, Vaskoska M, Vermue M H, Wijffels R H. Growth of Tetraselmis suecica in a tubular photobioreactor on wastewater from a fish farm. Water Res, 2014; 65: 290–296..

Di Termini I, Prassone A, Cattaneo C, Rovatti M. On the nitrogen and phosphorus removal in algal photobioreactors. Ecological Engineering, 2011; 37(6): 976–980.

Arbib Z, Ruiz J, Alvarez-Diaz P, Garrido-Perez C, Barragan J, Perales J A. Long term outdoor operation of a tubular airlift pilot photobioreactor and a high rate algal pond as tertiary treatment of urban wastewater. Ecological Engineering, 2013; 52: 143–153.

Menke S, Sennhenn A, Sachse J H, Majewski E, Huchzermeyer B, Rath T. Screening of microalgae for feasible mass production in industrial hypersaline wastewater using disposable bioreactors. CLEAN - Soil, Air, Water, 2012; 40(12): 1401–1407.

Chinnasamy S, Bhatnagar A, Claxton R, Das K C. Biomass and bioenergy production potential of microalgae consortium in open and closed bioreactors using untreated carpet industry effluent as growth medium. Bioresour Technol, 2010; 101(17): 6751–6760.

Wang T Y, Liu H C, Lee Y. Use of Anthropic Acclimated Spirulina platensis (Arthrospira platensis) Bio-adsorption in the Treatment of Swine Farm Wastewater. International Journal of Agriculture & Biology, 2013; 15(1): 107–112.

Zhu L, Hiltunen E, Shu Q, Zhou W, Li Z, Wang Z. Biodiesel production from algae cultivated in winter with artificial wastewater through pH regulation by acetic acid. Applied Energy, 2014; 128: 103–110.

Cicci A, Stoller M, Bravi M. Microalgal biomass production by using ultra- and nanofiltration membrane fractions of olive mill wastewater. Water Res, 2013; 47(13): 4710–4718.

Ruiz-Martinez A, Martin Garcia N, Romero I, Seco A, Ferrer J. Microalgae cultivation in wastewater: Nutrient removal from anaerobic membrane bioreactor effluent. Bioresour Technol, 2012; 126: 247–253.

Maroneze M M, Barin J S, Menezes C R D, Queiroz M I, Zepka L Q, Jacob-Lopes E. Treatment of cattle-slaughterhouse wastewater and the reuse of sludge for biodiesel production by microalgal heterotrophic bioreactors. Scientia Agricola, 2014; 71(6): 521–524.

Shi J, Podola B, Melkonian M. Application of a prototype-scale Twin-Layer photobioreactor for effective N and P removal from different process stages of municipal wastewater by immobilized microalgae. Bioresour Technol, 2014; 154: 260–266.

Xin C, Addy M M, Zhao J, Cheng Y, Cheng S, Mu D, et al. Comprehensive techno-economic analysis of wastewater-based algal biofuel production: A case study. Bioresour Technol, 2016; 211: 584–593.

Min M, Hu B, Mohr MJ, Shi A, Ding J, Sun Y, et al. Swine manure-based pilot-scale algal biomass production system for fuel production and wastewater treatment: A case study. Appl Biochem Biotechnol, 2014; 172(3): 1390–1406.

Filippino K C, Mulholland M R, Bott C B. Phycoremediation strategies for rapid tertiary nutrient removal in a waste stream. Algal Research, 2015; 11: 125–133.

Babatsouli P, Fodelianakis S, Paranychianakis N, Venieri D, Dialynas M, Kalogerakis N. Single stage treatment of saline wastewater with marine bacterial-microalgae consortia in a fixed-bed photobioreactor. J Hazard Mater, 2015; 292: 155–163.

Singh S K, Bansal A, Jha M K, Dey A. An integrated approach to remove Cr(VI) using immobilized Chlorella minutissima grown in nutrient rich sewage wastewater. Bioresour Technol, 2012; 104: 257–265.

Ruiz-Marin A, Mendoza-Espinosa L G, Stephenson T. Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. Bioresour Technol, 2010; 101(1): 58–64.

Zhang E, Wang B, Wang Q, Zhang S, Zhao B. Ammonia-nitrogen and orthophosphate removal by immobilized Scenedesmus sp. isolated from municipal wastewater for potential use in tertiary treatment. Bioresour Technol, 2008; 99(9): 3787–3793.

Gao F, Yang Z H, Li C, Zeng G M, Ma D H, Zhou L. A novel algal biofilm membrane photobioreactor for attached microalgae growth and nutrients removal from secondary effluent. Bioresour Technol, 2015; 179: 8–12.

Boelee N C, Temmink H, Janssen M, Buisman C J N, Wijffels R H. Balancing the organic load and light supply in symbiotic microalgal–bacterial biofilm reactors treating synthetic municipal wastewater. Ecological Engineering, 2014; 64: 213–221.

Arbib Z, Ruiz J, Alvarez-Diaz P, Garrido-Perez C, Perales J A. Capability of different microalgae species for phytoremediation processes: Wastewater tertiary treatment, CO2 bio-fixation and low cost biofuels production. Water Research, 2014; 49: 465–474.

Christenson L B, Sims R C. Rotating algal biofilm reactor and spool harvester for wastewater treatment with biofuels by-products. Biotechnology and Bioengineering, 2012; 109(7): 1674–1684.

Praveen P, Loh K C. Nitrogen and phosphorus removal from tertiary wastewater in an osmotic membrane photobioreactor. Bioresour Technol, 2016; 206: 180–187.

Gao F, Li C, Yang Z H, Zeng G M, Mu J, Liu M, et al. Removal of nutrients, organic matter, and metal from domestic secondary effluent through microalgae cultivation in a membrane photobioreactor. Journal of Chemical Technology & Biotechnology, 2016: 2713–2719.

Sukacova K, Trtilek M, Rataj T. Phosphorus removal using a microalgal biofilm in a new biofilm photobioreactor for tertiary wastewater treatment. Water Res, 2015; 71: 55–63.

Boonchai R, Seo G. Microalgae membrane photobioreactor for further removal of nitrogen and phosphorus from secondary sewage effluent. Korean Journal of Chemical Engineering, 2015; 32(10): 2047–2052.

Gao F, Yang Z H, Li C, Wang Y J, Jin W H, Deng Y B. Concentrated microalgae cultivation in treated sewage by membrane photobioreactor operated in batch flow mode. Bioresour Technol, 2014; 167: 441–446.

Nwoba E G, Ayre J M, Moheimani N R, Ubi B E, Ogbonna J C. Growth comparison of microalgae in tubular photobioreactor and open pond for treating anaerobic digestion piggery effluent. Algal Research, 2016; 17: 268–276.

de Godos I, Blanco S, Garcia-Encina P A, Becares E, Munoz R. Influence of flue gas sparging on the performance of high rate algae ponds treating agro-industrial wastewaters. Journal of Hazardous Materials, 2010; 179(1-3): 1049–1054.

Essam T, ElRakaiby M, Hashem A. Photosynthetic based algal-bacterial combined treatment of mixtures of organic pollutants and CO2 mitigation in a continuous photobioreactor. World Journal of Microbiology and Biotechnology, 2013; 29(6): 969–974.

Van Den Hende S, Vervaeren H, Desmet S, Boon N. Bioflocculation of microalgae and bacteria combined with flue gas to improve sewage treatment. N Biotechnol, 2011; 29(1): 23–31.

de-Bashan LE, Bashan Y. Immobilized microalgae for removing pollutants: review of practical aspects. Bioresour Technol, 2010; 101(6): 1611–1627.

Tampion J, Tampion M D. Immobilized cells : principles and applications: Cambridge University Press, 1987.

Mallick N, Rai L. Removal of inorganic ions from wastewaters by immobilized microalgae. World Journal of Microbiology and Biotechnology, 1994; 10(4): 439–443.

Travieso L, Benitez F, Dupeiron R. Sewage treatment using immobilied microalgae. Bioresour Technol, 1992; 40(2): 183–187.

Tam N, Wong Y. Effect of immobilized microalgal bead concentrations on wastewater nutrient removal. Environmental Pollution, 2000; 107(1): 145–151.

Mallick N. Biotechnological potential of immobilized algae for wastewater N, P and metal removal: a review. biometals, 2002; 15(4): 377–390.

Zamalloa C, Boon N, Verstraete W. Decentralized two-stage sewage treatment by chemical-biological flocculation combined with microalgae biofilm for nutrient immobilization in a roof installed parallel plate reactor. Bioresour Technol, 2013; 130: 152–160.

Travieso L, Benitez F, Weiland P, Sanchez E, Dupeyron R, Dominguez A. Experiments on immobilization of microalgae for nutrient removal in wastewater treatments. Bioresour Technol, 1996; 55(3): 181–186.

Roeselers G, Loosdrecht M C, Muyzer G. Phototrophic biofilms and their potential applications. J Appl Phycol, 2008; 20(3): 227–235.

Kesaano M, Sims R C. Algal biofilm based technology for wastewater treatment. Algal Research, 2014; 5: 231–240.

Posadas E, Garcia-Encina P A, Soltau A, Dominguez A, Diaz I, Munoz R. Carbon and nutrient removal from centrates and domestic wastewater using algal-bacterial biofilm bioreactors. Bioresour Technol, 2013; 139: 50–58.

Bilad M R, Arafat H A, Vankelecom I F J. Membrane technology in microalgae cultivation and harvesting: A review. Biotechnology Advances, 2014; 32(7): 1283–1300.

Kumar A, Yuan X, Sahu A K, Ergas S J, Van Langenhove H, Dewulf J. A hollow fiber membrane photo-bioreactor for CO2 sequestration from combustion gas coupled with wastewater treatment: A process engineering approach. Journal of Chemical Technology and Biotechnology, 2010; 85(3): 387–394.

Xu M, Bernards M, Hu Z. Algae-facilitated chemical phosphorus removal during high-density Chlorella emersonii cultivation in a membrane bioreactor. Bioresour Technol, 2014; 153: 383–387.

Low S L, Ong S L, Ng H Y. Biodiesel production by microalgae cultivated using permeate from membrane bioreactors in continuous system. Water Sci Technol, 2014; 69(9): 1813–1819.

Marbelia L, Bilad M R, Passaris I, Discart V, Vandamme D, Beuckels A, et al. Membrane photobioreactors for integrated microalgae cultivation and nutrient remediation of membrane bioreactors effluent. Bioresour Technol, 2014; 163: 228–235.

Wang Y H, Wu C M, Wu W L, Chu C P, Chung Y J, Liao C S. Survey on nitrogen removal and membrane filtration characteristics of Chlorella vulgaris Beij. on treating domestic type wastewaters. Water Sci Technol, 2013; 68(3): 695–704.

Su Z F, Li X, Hu H Y, Wu Y H, Tsutomu N. Culture of Scenedesmus sp. LX1 in the modified effluent of a wastewater treatment plant of an electric factory by photo-membrane bioreactor. Bioresour Technol, 2011; 102(17): 7627–7632.

Singh G, Thomas P B. Nutrient removal from membrane bioreactor permeate using microalgae and in a microalgae membrane photoreactor. Bioresour Technol, 2012; 117: 80–85.

Choi H. Intensified production of microalgae and removal of nutrient using a microalgae membrane bioreactor (MMBR). Applied Biochemistry and Biotechnology, 2014; 175(4): 2195–2205.

Gao F, Li C, Yang Z H, Zeng G M, Feng L J, Liu J Z, et al. Continuous microalgae cultivation in aquaculture wastewater by a membrane photobioreactor for biomass production and nutrients removal. Ecological Engineering, 2016; 92: 55–61.

Matthijs H C P, Balke H, Hes U M V, Kroon B M A, Mur L R, Binot R A. Application of light-emitting diodes in bioreactors: Flashing light effects and energy economy in algal culture ( Chlorella pyrenoidosa ). Biotechnology & Bioengineering, 1996; 50(1): 98–107.

Degen J, Uebele A, Retze A, Schmid-Staiger U, Trösch W. A novel airlift photobioreactor with baffles for improved light utilization through the flashing light effect. Journal of Biotechnology, 2001; 92(2): 89–94.

Tanwar P, Nandy T, Ukey P, Manekar P. Correlating on-line monitoring parameters, pH, DO and ORP with nutrient removal in an intermittent cyclic process bioreactor system. Bioresource Technology, 2008; 99(16): 7630–7635.

Novoveská L, Zapata A K M, Zabolotney J B, Atwood M C, Sundstrom E R. Optimizing microalgae cultivation and wastewater treatment in large-scale offshore photobioreactors. Algal Research, 2016; 18: 86–94.

Wiley P, Harris L, Reinsch S, Tozzi S, Embaye T, Clark K, et al. Microalgae cultivation using offshore membrane enclosures for growing algae (OMEGA). Journal of Sustainable Bioenergy Systems, 2013; 3(1): 18–32.

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