Optimization of hydrogen production from agricultural wastes using mixture design

Liu Shuang, Wang Chunying, Yin Lili, Li Wenzhe, Wang Zhongjiang, Luo Lina


Abstract: Hydrogen production from food waste, cattle manure, potato pulp and pig manure was optimized through using mixture design in this study. The synergic and antagonistic effects of the four substrates on hydrogen yield, substrate conversion efficiency and pH were evaluated. The results showed that the optimal proportion of food waste, cattle manure, potato pulp and pig manure were 61.6%, 38.4%, 0, and 0, respectively. Under the optimal condition, hydrogen yield of 21.0 mL/g VS with VS reduction of 29.4% and pH of 5 could be obtained. The interaction between food waste and cattle manure had strongest synergistic effects. Hydrogen was mainly produced by acetic-butyric metabolic pathway, and ammonification of protein played an important role in the maintenance of pH.
Keywords: hydrogen, biohydrogen production, agricultural waste, dark fermentation, mixture design
DOI: 10.3965/j.ijabe.20171003.2688

Citation: Liu S, Wang C Y, Yin L L, Li W Z, Wang Z J, Luo L N. Optimization of hydrogen production from agricultural wastes using mixture design. Int J Agric & Biol Eng, 2017; 10(3): 246–254.


hydrogen, biohydrogen production, agricultural waste, dark fermentation, mixture design


Guo X M, Trably E, Latrille E, Carrère H, Steyer J P. Hydrogen production from agricultural waste by dark fermentation: A review. Int J Hydrogen Energy, 2010; 35(19): 10660–10673.

Lee Y W, Chung J. Bioproduction of hydrogen from food waste by pilot-scale combined hydrogen/methane fermentation. Int J Hydrogen Energy, 2010; 35(21), 11746–11755.

Mohan S V, Mohanakrishna G, Goud R K, Sarma P N. Acidogenic fermentation of vegetable based market waste to harness biohydrogen with simultaneous stabilization. Bioresour Technol, 2009; 100: 3061–3068.

Tenca A, Schievano A, Lonati S, Malagutti L, Oberti R, Adani F. Looking for practical tools to achieve next-future applicability of dark fermentation to produce bio-hydrogen from organic materials in continuously stirred tank reactors. Bioresour Technol, 2011; 102: 7910–7916.

Li W W, Yu H Q. Anaerobic Granule technologies for hydrogen recovery from wastes: The way forward. Critical Reviews in Environmental Science and Technology, 2013; 43: 12, 1246–1280.

Singh L, Wahid Z A. Methods for enhancing bio-hydrogen production from biological process: A review. Journal of Industrial and Engineering Chemistry, 2015; 21: 70–80.

Fang H H P, Liu H. Effect of pH on hydrogen production from glucose by a mixed culture. Bioresour Technol, 2002; 82, 87–93.

Van Ginkel S, Sung S, Lay J J. Biohydrogen production as a function of pH and substrate concentration. Environ Sci

Technol, 2001; 35: 4726–4730.

Zhu H G, Parker W, Basnar R, Proracki A, Falletta P, Béland M, et al. Buffer requirements for enhanced hydrogen production in acidogenic digestion of food wastes. Bioresour Technol, 2009; 100: 5097–5102.

Tenca A, Schievano A, Perazzolo F, Adani F, Oberti R. Biohydrogen from thermophilic co-fermentation of swine manure with fruit and vegetable waste: Maximizing stable production without pH control. Bioresour Technol, 2011; 102: 8582–8588.

Kim D H, Kim S H, Kim H W, Kim M S, Shin H S. Sewage sludge addition to food waste synergistically enhances hydrogen fermentation performance. Bioresour Technol, 2011; 102: 8501–8506.

Kim S H, Han S K, Shin H S. Feasibility of biohydrogen production by anaerobic co-digestion of food waste and sewage sludge. Int J Hydrogen Energy, 2004; 29(15): 1607–1616.

Fan Y T, Xing Y, Ma H C, Pan C M, Hou H W. Enhanced cellulose-hydrogen production from cornstalk by lesser panda manure. Int J Hydrogen Energy, 2008; 33(21): 6058–6065.

Do J S, Liou B C. A mixture design approach to optimizing the cathodic compositions of proton exchange membrane fuel cell. J Power Sources, 2011; 196: 1864–1871.

Nikzade V, Tehrani M M, Saadatmand-Tarzjan M. Optimization of low-cholesterol-low-fat mayonnaise formulation: Effect of using soy milk and some stabilizer by a mixture design approach. Food Hydrocolloid, 2012; 28: 344–352.

Rao P V, Baral S S. Experimental design of mixture for the anaerobic co-digestion of sewage sludge. Chem Eng J, 2011; 172: 977–986.

Prakasham R S, Sathish T, Brahmaiah P, Subba C, Rao R S, Hobbs P J. Biohydrogen production from renewable agri-waste blend: Optimization using mixer design. Int J Hydrogen Energy, 2009; 34(15): 6143–6148.

Rossi D M, Berne da Costa J, Aquino de Souza E, Peralba M D C R, Samios D, Záchia Ayub M A. Comparison of different pretreatment methods for hydrogen production using environmental microbial consortia on residual glycerol from biodiesel. Int J Hydrogen Energy, 2011; 36: 4814–4819.

APHA. Standard methods for the examination of water and wastewater. 21st ed. Washington DC: American Water Work Association, and Water Pollution Control Federation, 2004.

Cornell J A. Experiments with mixtures: Designs, models, and the analysis of mixture data. 3rd ed. New York: John Wiley and Sons Inc., 2002.

Itoh Y, Tada K, Kanno T, Horiuchi J I. Selective production of lactic acid in continuous anaerobic acidogenesis by extremely low pH operation. J Biosci Bioeng, 2012; 114: 537–539.

Lay C H, Lin H C, Sen B, Chu C Y, Lin C Y. Simultaneous hydrogen and ethanol production from sweet potato via dark fermentation. J Clean Prod, 2012; 27: 155–164.

Zhu H G, Stadnyk A, Béland M, Seto P. Co-production of hydrogen and methane from potato waste using a two-stage anaerobic digestion process. Bioresour Technol, 2008; 99: 5078–5084.

Cuetos M J, Gómez X, Escapa A, Morán A. Evaluation and simultaneous optimization of bio-hydrogen production using 32 factorial design and the desirability function. J Power Sources, 2007; 169: 131–139.

Infantes D, González del Campo A, Villaseñor J, Fernández F J. Influence of pH, temperature and volatile fatty acids on hydrogen production by acidogenic fermentation. Int J Hydrogen Energy, 2011; 36: 15595–15601.

Ghimire A, Frunzo L, Pirozzi F, Trably E, Escudie R, Lens P

N L, et al. A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products. Applied Energy, 2015; 144: 73–95.

Horiuchi J I, Shimizu T, Tada K, Kanno T, Kobayashi M. Selctive production of organic acids in anaerobic acid reactor by pH control. Bioresour Technol, 2002; 82: 209–213.

Zoetemeyer R J, Van den Heuvel J C, Cohen A. pH influence on acidogenic dissimilation of glucous in an anaerobic digestor. Water Res, 1982; 16: 303–311.

Yokoyama H, Waki M, Ogino A, Ohmori H, Tanaka Y. Hydrogen fermentation properties of undiluted cow dung. J Biosci Bioeng, 2007; 104: 82–85.

Wang K S, Chen J H, Huang Y H, Huang S L, Baral S S. Integrated Taguchi method and response surface methodology to confirm hydrogen production by anaerobic fermentation of cow manure. Int J Hydrogen Energy, 2012; 38(1): 45–53.

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