Differentiating between fertilized and unfertilized eggs prior to incubation based on oxygen flux measurement

Wang Qiaohua, Fu Dandan, Ma Meihu, Zhang Tao

Abstract


One unresolved challenge in the egg industry is how to efficiently and non-invasively detect unfertilized eggs prior to incubation. This detection ability would not only significantly improve hatching rates and reduce costs but also conserve incubator space and prevent poor-quality embryos from contributing to the spread of infections. This study demonstrates a procedure for distinguishing between fertilized and unfertilized eggs prior to incubation by studying the respiratory differences between fertilized and unfertilized eggs using the Non-invasive Micro-test Technique (NMT). A customized micro-testing examination platform, NMT Egg Testing System (NMT-ETS) was constructed for the real-time monitoring of the intensity and rate of oxygen exchange between the egg and its external environment. The results from this study revealed that at room temperature, there is a significant difference in gas exchange rates between fertilized and unfertilized eggs. The results indicate that the oxygen flux of fertilized eggs exceed 20 pmol/(cm2•s), whereas unfertilized eggs show a much lower oxygen flux. Based on the results, the NMT method can be used to effectively distinguish between fertilized and unfertilized chicken eggs.
Keywords: oxygen flux, gas exchange rates, fertilized eggs, unfertilized eggs, non-invasive micro-test technique
DOI: 10.25165/j.ijabe.20171004.2606

Citation: Wang Q H, Fu D D, Ma M H, Zhang T. Differentiating between fertilized and unfertilized eggs prior to incubation based on oxygen flux measurement. Int J Agric & Biol Eng, 2017; 10(4): 243–251.

Keywords


oxygen flux, gas exchange rates, fertilized eggs, unfertilized eggs, non-invasive micro-test technique

References


Ma M H. Processing technology and quality safety control strategy of egg products. China Poultry, 2009; 31: 1–5. (in Chinese)

Joseph M M. Factors affecting the hatchability and hatching. China Poultry, 2002; 24: 27–32. (in Chinese)

Das K, Evans M D. Detecting fertility of hatching eggs using machine vision II: Neural network classifiers. Transactions of the ASAE, 1992; 35: 2035–2041.

Bamelis F R, Tona K, de Baerdemeaker J G. Detection of early embryonic development in chicken eggs using visible light transmission. British Poultry Science, 2002; 43: 204–212.

Smith D P, Lawewnce K C, Park B. Detection of fertility and early development of hatching eggs with hyperspectral imaging. Proc. 11th European Symposium on the Quality of Eggs and Egg Products Netherlands: World’s Poultry Science Association, 2005; pp.176–180.

Jones S T, Shattuck R E. Detection of Early Embryonic Development in hatching eggs: a hyperspectral imaging systems and neural network approach. Johns Hopkins Applied Physics Laboratory Technical Digest, 2005; 1: 67–73.

Bamelis F, Kemps B, Mertens K, Tona K, de Ketelaere B, Decuypere E, et al. Non-destructive measurements on eggs during incubation. Avian and Poultry Biology Reviews, 2004; 15: 3–4.

Liu L, Ngadi M. Detecting fertility and early embryo development of chicken eggs using near-infrared hyper-spectral imaging. Food and Bio-process Technology, 2013; 6: 9.

Kerstin W A. Seymour R S. Effect of regional changes to shell conductance on oxygen consumption and growth of chicken embryos. Respiration Physiology, 2002; 129(3): 385–395.

Sunder L M,Tompkins D M, Hudson P J. The role of oxygen availability in the embryo nation of Heterakis gallinarum eggs. International Journal for Parasitology, 2000; 30: 1481–1485.

Pearson J T, Haque M A, Hou P C, Tazawa H. Developmental patterns of O2 consumption, heart rate and O2 pulse in unturned eggs. Respiration Physiology, 1996; 103: 83–87.

Seymour R S, Kerstn W A. Non-invasive measurement of oxygen partial pressure, lateral diffusion and chorioallantoic blood flow under the avian eggshell. Comparative Bio-chemistry and Physiology, Part A, 2008; 150: 258–264.

Kuhtreiber W M, Laffe L F. Detection of extracelluar calcium gradients with a calcium-specific vibrating electrode. Journal of Cell Biology, 1990; 110: 1565–1573.

Bakst M R, Gupta S K, Potts W, Akuffo V. Gross appearance of the turkey blastoderm at oviposition. Poultry Science, 1998; 77(8): 1228–1233.

Wang Q H, Zhang T, Ma M H. Oxygen respiratory regularity of eggs in storage period monitoring by non-invasive micro-test technique. Transactions of the CSAE, 2014; 3(5): 255–261.

Chwalibog A, Tauson A H, Ali A, Matthiesen C, Thorhayge K, Thorbek G. Gas exchange, heat production and oxidation of fat in chicken embryos from a fast or slow growing line. Comparative Biochemistry and Physiology, Part A, 2007; 146: 305–309.

Jacopo P M, Katherine L. Oxygen consumption of the chicken embryo: interaction between temperature and oxygenation, science direct. Respiratory Physiology & Neurobiology, 2005; 146: 97–106.

Wang Q H, Zhang T, Ma M H, Li X M. Study on the Relationship between egg O2 respiration and eggshell ultra structure. Advance Journal of Food Science and Technology, 2015; 9(3): 159–166.


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