Performance of alkaline pretreatment on pathogens inactivation and sludge solubilization

Yin Fubin, Li Zifu, Mayiani Saino, Dong Hongmin

Abstract


Inactivation of pathogen indicators (i.e., faecal coliforms, Salmonella spp., faecal streptococcus, and helminth eggs) were investigated during alkaline pretreatment (pH=10 and 12) in this study. The performance of alkaline pretreatment on the inactivation pathogens, kinetic of pathogens inactivation and sludge solubilization was evaluated. Results of alkaline pretreatment showed that the complete inactivation periods of pathogens time were 1.5 d, 1.5 d, 2 d, 2.5 d, 3 d, 3 d and 3 d for faecal sludge total solids (TS) of 1%, 2%, 4%, 6%, 8%, 10% and 12%, respectively. The kinetics of pathogen inactivation can be predicted better by Weibull than the first-order model. Meanwhile, the relationship between alkaline pretreatment time and the TS content of the sludge agrees with the exponential equation (y =1.3543e10.002x, 1% ≤ x ≤ 8%) and logarithmic equation (y =3, 8% ≤ x ≤ 12%). Furthermore, alkaline pretreatment can improve sludge solubilization and has a more significant effect on protein solubilization than on soluble chemical oxygen demand (SCOD).
Keywords: faecal sludge, alkaline pretreatment, pathogen inactivation, kinetic, sludge solubilization
DOI: 10.3965/j.ijabe.20171002.2600

Citation: Yin F B, Li Z F, Mayiani S, Dong H M. Performance of alkaline pretreatment on pathogens inactivation and sludge solubilization. Int J Agric & Biol Eng, 2017; 10(2): 216–223.

Keywords


faecal sludge, alkaline pretreatment, pathogen inactivation, kinetic, sludge solubilization

References


Lu J, Gavala H N, Skiadas I V, Mladenovska Z, Ahring B K. Improving anaerobic sewage sludge digestion by implementation of a hyper-thermophilic prehydrolysis step. Journal of Environmental Management, 2008; 88(4): 881–889.

Wang H L, Brown S L, Magesan G N, Slade A H, Quintern M, Clinton P W, et al. Technological options for the management of biosolids. Environmental Science and Pollution Research, 2008; 15(4): 308–317.

Taleghani G, Shabani K A. Technical-economical analysis of the Saveh biogas power plant. Renewable Energy, 2005; 30(3): 441–446.

Dinuccio E, Balsari P, Gioelli F, Menardo S. Evaluation of

the biogas productivity potential of some Italian agro-industrial biomasses. Bioresource Technology, 2010; 101(10): 3780–3783.

Sidhu J P S, Toze S G. Human pathogens and their indicators in biosolids: A literature review. Environment International, 2009; 35(1): 187–201.

Mackie Jensen P K, Phuc P D, Knudsen L G, Konradsen F. Hygiene versus fertiliser: The use of human excreta in agriculture - A Vietnamese example. International Journal of Hygiene and Environmental Health, 2008; 211(3-4): 432–439.

Chen Y, Fu B, Wang Y, Jiang Q, Liu H. Reactor performance and bacterial pathogen removal in response to sludge retention time in a mesophilic anaerobic digester treating sewage sludge. Bioresource Technology, 2012; 106: 20–26.

Forster-Carneiro T, Riau V, Pérez M. Mesophilic anaerobic digestion of sewage sludge to obtain class B biosolids: Microbiological methods development. Biomass and Bioenergy, 2010; 34(12): 1805–1812.

Astals S, Venegas C, Peces M, Jofre J, Lucena F, Mata A J. Balancing hygienization and anaerobic digestion of raw sewage sludge. Water Research, 2012; 46(19): 6218–6227.

Sahinkaya S, Sevimli M F. Synergistic effects of sono-alkaline pretreatment on anaerobic biodegradability of waste activated sludge. Journal of Industrial and Engineering Chemistry. 2013; 19(1): 197–206.

Manafi M. New developments in chromogenic and fluorogenic culture media. International Journal of Food Microbiology, 2000; 60: 205–218.

Rambach A. New plate medium for facilitated differentiation of Salmonella spp. from Proteus spp. and other enteric bacteria. Applied and Environmental Microbiology, 1990; 56(1): 301–303. PMID 2310184.

Suresh P, Rehg J. Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces. J Clin Microbiol, 1996; 34(1): 38–40.

Apha. Standard methods for the examination of water and wastewater, twentieth ed. Washington, DC: American public health association, 1998.

Lowry O H, Rosebrough N J, Farr A L, Randall R J. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 1951; 193(1): 265–275.

Buzrul S, Alpas H. Modeling inactivation kinetics of food borne pathogens at a constant temperature. LWT-Food Science and Technology, 2007; 40(4): 632–637.

Popat S C, Yates M V, Deshusses M A. Kinetics of inactivation of indicator pathogens during thermophilic anaerobic digestion. Water Research, 2010; 44(20): 5965–5972.

Chen H. Use of linear, Weibull, and log-logistic functions to

model pressure inactivation of seven foodborne pathogens in milk. Food Microbiology, 2007; 24(3): 197–204.

Fernández A, Salmerón C, Fernández P S, Martinez A. Application of a frequency distribution model to describe the thermal inactivation of two strains of Bacillus cereus. Trends in Food Science & Technology, 1999; 10(4-5): 158–162.

Linton R H, Carter W H, Pierson M D, Hackney C R. Use of a modified Gompertz equation to model nonlinear survival curves for Listeria monocytogenes Scott A. Journal of Food Protection, 1995; 58(9): 946–954.

Peleg M. On calculating sterility in thermal and non-thermal preservation methods. Food Research International, 1999; 32(4): 271–278.

Maizels R M, Bundy D A, Selkirk M E, Smith D F, Anderson R M. Immunological modulation and evasion by helminth parasites in human populations. Nature, 1993; 365(6449): 797–805.

Benyi X, Junxin L. Study on treatment of excess sludge under alkaline condition. Environmental Science, 2006; (02): 319–323.

Katsiris N, Alexandra K. Bound water content of biological sludges in relation to filtration and dewatering. Water Research, 1987; 21(11): 1319–1327.

Flores-Juarez C R, Rodríguez-García A, Cárdenas-Mijangos J, Montoya-Herrera L, Mora-Tovar L G, Bustos-Bustos E, et al. Chemically pretreating slaughterhouse solid waste to increase the efficiency of anaerobic digestion. Journal of Bioscience and Bioengineering, 2014; 118(4): 415–419.

van Boekel M A J S. On the use of the Weibull model to describe thermal inactivation of microbial vegetative cells. International Journal of Food Microbiology, 2002; 74(1-2): 139–159.

Uma Rani R, Adish Kumar S, Kaliappan S, Yeom I T, Rajesh Banu J. Low temperature thermo-chemical pretreatment of dairy waste activated sludge for anaerobic digestion process. Bioresource Technology, 2012; 103(1): 415–424.

Fdez. Güelfo L A, Álvarez-Gallego C, Márquez D S, Romero Garcia L I. The effect of different pretreatments on biomethanation kinetics of industrial organic fraction of municipal solid wastes (OFMSW). Chemical Engineering Journal, 2011; 171(2): 411–417.

Jang J-H, Ahn J-H. Effect of microwave pretreatment in presence of NaOH on mesophilic anaerobic digestion of thickened waste activated sludge. Bioresource Technology, 2013; 131: 437–442.

Chi Y, Li Y, Fei X, Wang S, Yuan H. Enhancement of thermophilic anaerobic digestion of thickened waste activated sludge by combined microwave and alkaline pretreatment. Journal of Environmental Science, 2011; 23(8): 1257–1265.


Full Text: PDF

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.